By James C. Taylor
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In the suspensions prepared above, the CB concentration was varied from 0 to 35 wt%. 3. 0845 rad and a plate diameter of 25 and/or 50 mm were used. Prior to the measurements, the suspensions were sheared at a rate 10 s1 for 2 min and then kept quiescently for 3 min. This pre-shear ensured a good reproducibility of the data. After a quiescent rest for 3 min, the measured G and G data did not change with time. Transmission electron microscope (TEM) observation was performed for solidified CB suspensions (for which the observation could be made in vacuum).
Coli) were needed to significantly inhibit EPS production (over 90%) without impacting bacterial growth. Figures 1 and 2 illustrate that the sub-MICs of BisBAL, BisEDT and BisPYR had negligible effects on the viability of E. coli (assessed using epifluorescence microscopy and atomic force microscopy) even after 2 days. The MICs summarized in Table 1 are consistent with values published by other researchers for Gramnegative bacteria including E. coli and S. marcescens[10, 50,52]. Additionally, subMIC of bismuth thiols were very effective at suppressing free and boundEPS of E.
Chemistry of Materials 2011, 23, 137-144. ; Sewing, K. , Studies on the mechanism of action of colloidal bismuth subcitrate I. Interaction with sulfhydryls. Pharmacology 1993, 47, 135-140.  Mahony, D. ; Hoffman, P. ; Briand, G. , Antimicrobial activities of synthetic bismuth compounds against Clostridium difficile. Antimicrobial Agents and Chemotherapy 1999, 43, (3), 582-588.  Stratton, C. ; Warner, R. ; Coudron, P. ; Lilly, N. , Bismuthmediated disruption of the glycocalyx-cell wall of Helicobacter pylori: ultrastructural evidence for a mechanism of action for bismuth salts.