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It could be observed that in vitro exposure for 24h to this immunotoxin was associated with the disappearance of monocytes from mononuclear cells (MNC) evaluated as RoMo-1-positive cells, whereas no selective removal of monocytes was observed in cultures exposed to RoMo-1 and dML I. 2). The use of toxic lectins partially denatured by means of molten salts might be a new variant for preparing immunotoxins. 8 Blood MNC (5 X 106/ml) were treated with IT, dMLI and RoMo-1 for 24h, washed and then cultured for 72h in the presence of PHA.
8 Blood MNC (5 X 106/ml) were treated with IT, dMLI and RoMo-1 for 24h, washed and then cultured for 72h in the presence of PHA. D. < 25 %, triplicates). Mol. wt. of IT is about 300,000. 5 Lectin/Sugar Interactions in Nonpolar Solvents Up to now lectin-sugar (glycoconjugate) interactions have been performed in aqueous systems. Pfiiller et al. (1989) investigated the reaction of ML I and other lectins (ricin, Lens culinaris lectin, Con A) with carbohydrates in iso-octane. They entrapped the leetins in reverse micelles formed by the anionic surfactant Aerosol OT (AOT) in iso-octane after addition of small amounts of water.
The authors point out that ML I, the main cytotoxic protein of Viscum album represents only a small part of the total cytotoxic pro· teins in lscado?. Sauviat (1990) detected that Ph oratoxin B, a toxin isolated from mistletoe has a depolarizing ac· tion on frog skeletal muscle fibres. This effect is based on increasing the resting membrane con· ductance. It seems to exist an interaction between Ca ++ and the toxin molecule at the membrane level. Perhaps the toxic one acts as a detergent, similar to cardiotoxins.