By Jean Effront
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Extra resources for biochemical catalysts in life and industry
Fluoride, like oxalate, is a decalcifying agent and it is this precipitation of the calcium ions that makes plasma non-coagulable. But why is it that on the final addition of CaCk, coagulation no longer takes place, as is the case with oxalated plasma? This difference comes from the fact that calcium fluoride, in being precipitated, carries with it, to a more or less extent, not only fibrinogen but also serozyme, the latter even more completely than fibrinogen. c. c. c. T % sodium oxalate solution.
BLOOD PLASMAS NOT SPONTANEOUSLY COAGULABLE. In the preceding chapter we became familiar with the principal constituents which come into play in the phenomenon of the coagulation of blood. We have studied thrombin and fibrinogen, and established the relation which exists between the two. We now return to the question and, excluding the theories which we have just described, will try to penetrate the actual mechanism of coagulation by analytical methods. Let us ask first of all why the blood, liquid in the blood-vessels, coagulates on leaving them.
The tubes are allowed to stand for several hours, then the two mixtures are centrifuged and the clear 56 BIOCHEMICAL CATALYSTS liquids decanted. I t is then found that the first liquid (A), which has been in contact with calcium fluoride, with addition of CaCl 2 or thrombin, does not coagulate, and when heated to 6o°-65 o 5 it is no longer turbid. The fibrinogen as well as the active substance has been carried down by the precipitate. On the other hand, the second liquid (B), which has not been in contact with the calcium fluoride, is coagulated by addition of CaClo, or of serum, and when heated to 6o°-65° becomes very turbid.