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Int J Environ Sci Technol 11(7):2087–2094 8. Schink B (1997) Energetics of syntrophic cooperation in methanogenic degradation. Microbiol Mol Biol Rev 61(2):262–280 9. Stams AJM, Plugge CM (2009) Electron transfer in syntrophic communities of anaerobic bacteria and archaea. Nat Rev Microbiol 7(8):568–577 10. McInerney MJ, Sieber JR, Gunsalus RP (2009) Syntrophy in anaerobic global carbon cycles. Curr Opin Biotechnol 20(6):623–632 11. Worm P, Koehorst JJ, Visser M, Sedano-Núñez VT, Schaap PJ, Plugge CM, Sousa DZ, Stams AJM (2014) A genomic view on syntrophic versus non-syntrophic lifestyle in anaerobic fatty acid degrading communities.
Biological structures can be maintained by critical point drying, lyophilisation or high-pressure freezing. Instead of SEM, environmental SEM using lower vacuum pressures can be used alternatively as well. Pinpoint extraction and ultra-thin layer observations by consecutive cryosectioning are further techniques to investigate certain regions of interest and cellular aspects respectively based on SEM/TEM or focused ion beam (FIB)-SEM that can be also combined with CLSM 3D imaging for real 3D correlations of one and the same biological event in an identical sample [221, 222].
Zebulun O (2013) Molecular and biological techniques used in landﬁll investigations: A mini-review. Biotechnol Mol Biol Rev 8(2):35–42 192. Lee N, Nielsen PH, Andreasen KH, Juretschko S, Nielsen JL, Schleifer KH, Wagner M (1999) Combination of fluorescent in situ hybridization and microautoradiography-a new tool for structure-function analyses in microbial ecology. Appl Environ Microbiol 65(3):1289– 1297 193. Nielsen JL, Nielsen PH (2010) Combined microautoradiography and fluorescence in situ hybridization (MAR-FISH) for the identiﬁcation of metabolically active microorganisms.